Protein AKT map
PMID:18215152
PMID:17141160
PMID:16962829
PMID:17043309
PMID:10801415
Activation of AKT by mTORC2 and PDK1:
From Jacinto, 2008 (PMID:18215152); In vivo analyses also support mTORC2 as a critical regulator of HM phosphorylation. Knockdown of the mTORC2 component rictor, but not the mTORC1 component raptor, led to defective phosphorylation of Akt at Ser473 [39]. This decrease in Ser473 phosphorylation was also accompanied by defective Thr308 phosphorylation, in agreement with previous claims that Ser473 phosphorylation is important for subsequent Thr308 phosphorylation [168,176,177]. Genetic studies using MEFs from mice that have deficient mTORC components confirm that mTORC2 is required for HM site phosphorylation. In rictor-, mLST8- or SIN1-knockout cells, Ser473 phosphorylation was abolished. Phosphorylation at Thr308 was not grossly abnormal in rictor-/- cells [51,52] and was normal in mLST8-/- [27] and in SIN1-/- cells [40]. These findings support the alternative view that PDK1 docking to the phosphorylated HM site of Akt is not critical for T-loop phosphorylation (see also S6K and PKC). Studies from PDK1-/- ES cells have also revealed that in the absence of Thr308 phosphorylation, the HM site phosphorylation at Ser473 still occurs [146]. Thus phosphorylation of the T-loop and HM site of Akt seems to occur independently of each other. Akt remained partly active in mTORC2-disrupted cells and phosphorylated a subset of its known substrates but was defective in the phosphorylation of the apoptosis promoting proteins Foxo1/3a [40]. These findings suggest that the phosphorylation of Ser473 may play a role in cell survival and that HM site phosphorylation may regulate substrate specificity in Akt and possibly other AGC kinases as well.
Modifications:
In compartment: Cell membrane
  1. AKT@Cell membrane map
  2. AKT|​T450_pho@Cell membrane map
  3. AKT|​T450_pho@Cell membrane map
  4. AKT|​S473_pho|​T450_pho|​active@Cell membrane map
Participates in complexes:
In compartment: Cell membrane
  1. AKT:​HSP90@Cell membrane map
  2. AKT:​PI3,4,5-P_sub_3@Cell membrane map
  3. AKT|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  4. AKT|​S473_pho|​T450_pho:​Sin1.1@Cell membrane map
  5. AKT|​S473_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  6. AKT|​T308_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  7. AKT|​T308_pho|​S473_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
Participates in reactions:
As Reactant or Product:
  1. AKT|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map → map AKT|​S473_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  2. AKT|​T450_pho@Cell membrane map → map AKT|​T450_pho@Cell membrane map
  3. AKT|​T450_pho@Cell membrane map + PI3,4,5-P_sub_3@Cell membrane map → map AKT|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  4. AKT|​S473_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map → map AKT|​T308_pho|​S473_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  5. PDGF:​PDGFR_beta@Cell membrane map → map AKT|​T308_pho|​S473_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  6. AKT|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map → map AKT|​S473_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  7. AKT|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map → map AKT|​T308_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map
  8. rAKT@Cell membrane map → map AKT@Cell membrane map
  9. AKT@Cell membrane map → map AKT|​T450_pho@Cell membrane map
  10. AKT@Cell membrane map → map degraded
  11. HSP90@Cell membrane map + AKT@Cell membrane map → map AKT:​HSP90@Cell membrane map
  12. AKT|​T450_pho@Cell membrane map → map AKT|​S473_pho|​T450_pho|​active@Cell membrane map
  13. AKT|​S473_pho|​T450_pho|​active@Cell membrane map + Sin1.1@Cell membrane map → map AKT|​S473_pho|​T450_pho:​Sin1.1@Cell membrane map
  14. AKT|​S473_pho|​T450_pho|​active@Cell membrane map → map AKT|​T450_pho@Cell membrane map
  15. AKT|​T308_pho|​S473_pho|​T450_pho:​PI3,4,5-P_sub_3@Cell membrane map → map AKT:​PI3,4,5-P_sub_3@Cell membrane map
As Catalyser:
  1. TSC2|​S1798_pho@Nucleus map → map TSC2|​S981_pho|​S939_pho|​S1798_pho|​T1462_pho@Cell membrane map
  2. GSK3alpha/beta@Cell membrane map → map GSK3alpha/beta|​S21/9_pho@Cell membrane map
  3. TSC1:​TSC2@Endomembrane map → map TSC1:​TSC2|​S981_pho|​S939_pho|​T1462_pho@Endomembrane map
  4. PRAS40:​mTORC1@Cell membrane map → map PRAS40|​T246_pho:​mTORC1@Cell membrane map
  5. AKT@Cell membrane map → map degraded
  6. FoxO1/3a@Nucleus map → map FoxO1/3a|​S253_pho|​T24/32_pho@Nucleus map
  7. p27|​S10_pho@Cell membrane map → map p27|​S10_pho|​T198_pho@Cell membrane map
  8. p27|​S10_pho|​T198_pho@Cell membrane map → map p27|​S10_pho|​T198_pho|​T157_pho@Cell membrane map
  9. Mdm2@Cell membrane map → map Mdm2|​pho@Cell membrane map